* SLAN® Real-Time PCR * Smartcycles II * Applied 73* ABI * ABI FAST * ABI * AB Step One * AB Step One Plus * Agilent MxP CFX96 CFX * ExiCyclerTM 96 * iQ5 MyiQ Cycler * Illumina Eco * LightCycler Nano Total: 10,5 μL . Principle of Real-Time PCR The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal. Master Mix with the HT Fast Real-Time PCR System from Applied Biosystems. For detailed instructions, refer to the full product manual. Prepare the Reactions 1 Dilute the reference dye using nuclease-free PCR-grade water. 2 Prepare the experimental reactions by combining the components of the.
The HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines and well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability. The PCR process and 5' nuclease process are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. Applied Biosystems, ABI PRISM, GeneAmp, MicroAmp and VIC are registered trademarks and AB (Design), Applera, FAM, JOE, NED, Primer Express, ROX,TAMRA and TET are. DRAFT September 1, am, www.doorway.ru vi Applied Biosystems HT Fast Real-Time PCR System and SDS Enterprise Database User Guide Chapter 6 Analyzing Real-Time Data.
Real-time PCR continues to have a major impact across many plastic reaction vessels (e.g. ABI , ABI HT, DNA Engine. USER GUIDE. Getting Started with QuantStudio™ 6 and 7 Flex. Real-Time PCR System Software Experiments. Booklet 1. Publication Number Revision A. This manual is written for principal investigators and laboratory staff Safety Refer to the Applied Biosystems // Fast Real-Time PCR System.
0コメント